The FX500 is a microfluidics chip based cell sorter. Its fully replaceable fluidics system lets researchers easily replace the entire fluidics path to control sample-to-sample carryover. The FX500 features comprehensive fluidics controls, advanced automation and easy to use software.
Patented CoreFinder? technology automates instrument setup and streamlines workflow. The optical design offers up to three collinear excitation lasers - 488 nm, 561 nm, and 638 nm and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser-based on filter setting.
The FX500 is for non-clinical research use only and is a Class 1 laser product.
The FX500 features a unique replaceable fluidics system which includes an E-beam irradiated sorting chip and PEEK sample line, as well as a gamma irradiated sheath line assembly.
These single use consumables can be easily exchanged between experimental runs to control the sample-to-sample carryover.
The PEEK sample line introduces the sample into the E-beam irradiated sorting chip. Exchanging these single-use consumables between experiments controls sample-to-sample carryover.
The sheath line assembly is made of gamma irradiated sheath line that can be linked to multiple sheath bags and connected to the chip using the chip connector.
The absence of fluidics pumps and inline valves in this design allows for an easy replacement of the sheath line as needed
A unique pressurized stainless sheath tank encloses a removable rack where sheath bags can be mounted. The sheath fluid from all the bags is channeled from a single sheath line to the chip.
The E-beam irradiated sorting chip acts as an integrated flow cell/nozzle and can be easily installed and removed.
The sample and sheath fluid are injected into the chip's micro-fluidic channels from inlet ports. Excitation lasers interrogate the sample within the chip, and then the sample it passes through, and is sorted as droplets. The chip is directly connected to the waste line and can be declogged using software commands.
Unlike traditional systems that require nozzles to be removed for manual cleaning, the FX500 system uses an automated purge and prime cycle triggered by the software to clear the chip. A chip can be used continuously without removing it. The chip is available in 70-μm and 100-μm allowing researchers to easily adapt the system to various cell sizes.